The ability of the TOF.SIMS 5 to image individual molecular compounds in order to obtain their detailed spatial arrangement was used to study the degenerative/regenerative processes in the muscles of a dystrophin-deficient model mouse.
The specific distribution of different substances (fatty acids, vitamin E, triglycerides, phosphatidic acids, coenzyme Q9 and chlorine) were imaged from untreated mouse leg sections.
The images of individual substances show their distribution over the section.
A concentration of chlorine is found in the destructured zone, known from other analyses to be regenerating. The chlorine is probably combined with sodium and potassium also found in this zone.
Fatty acids and triglycerides are located mainly in the blue intermediate zone, vitamin E, phosphatidic acids, and coenzyme Q9 are found in both the blue and green intermediate zones.
But there is much more vitamin E and coenzyme Q9 in the green zone than in the blue, and it is suggested that these high accumulations mark oxidation stress and inflammatory reactions which can lead to muscle necrosis. Both zones are under oxidation stress and the green zone can be considered degenerative.
It was also found that the ratio of fatty acids, palmitic acid to palmitoic acid, and the ratio of stearic acid to oleic acid varied between the zones.
Overlay: Cl, Oleic Acid, Coenzyme Q9
The 500 x 500 µm2 overlay image of several ion maps of the mouse leg section shows an apparently healthy zone on the right (dark red/black), a destructured zone (red) on the left corresponding to that seen in the optical image, and two intermediate zones (green and blue)
The data was provided by Dr A. Brunelle, ICSN-CNRS, Gif-sur-Yvette, France. The reference is: David Touboul, Alain Brunelle, Frédéric Halgand, Sabine De La Porte and Olivier Laprévote 2005, Journal of Lipid Research, Vol. 46, 1388-1395, July 2005